When it comes to preserving your dog’s tissue for cloning, how you store the sample can make all the difference. The viability of your pet’s cells depends on proper handling and storage from the moment the biopsy is collected. Whether you’re preparing for cloning now or simply want the option in the future, here are the essential do’s and don’ts for storing your dog’s tissue correctly.
✅ DO: Use a Professional Biopsy Kit
One of the most important steps is starting with a certified biopsy kit designed specifically for tissue preservation. These kits are made to maintain the integrity of your pet’s cells until they reach the lab.
Why it matters:
Improper containers, temperature variations, or contamination can render the sample unusable, making cloning impossible.
✅ DO: Store the Sample Cold — But Not Frozen
Once the sample is collected, it must be kept cold (refrigerated), not frozen. Freezing can destroy the cell structure, making the sample non-viable for cloning.
Storage tip:
Store the sample at around 4°C / 39°F, ideally in a refrigerator or insulated cooler with cold packs.
✅ DO: Ship the Sample Promptly
Time is critical. After collecting the sample, it should be shipped to the preservation or cloning facility within 24–48 hours.
Why:
Delays increase the chance of cell degradation. The sooner it’s processed in a lab, the higher the chances of successful cell culture and preservation.
✅ DO: Follow Sterile Protocols
Always follow sterile techniques during the biopsy collection. The tissue must remain uncontaminated by bacteria, dirt, or foreign materials.
Pro tip:
If you’re unsure how to handle the biopsy, request your veterinarian to perform it using your cloning company’s biopsy kit instructions.
❌ DON’T: Use Improvised Containers or Household Supplies
Avoid storing the tissue in random containers like Tupperware, plastic bags, or homemade solutions. These materials are not sterile or temperature-controlled.
Result:
Such practices can cause contamination or cellular breakdown, leading to sample rejection.
❌ DON’T: Delay Refrigeration
Leaving the tissue at room temperature — even for a few hours — can damage the sample.
Tip:
Have your cold storage method ready before the biopsy procedure begins.
❌ DON’T: Freeze the Tissue
Freezing causes ice crystals to form inside cells, rupturing them and making the tissue unusable for cloning.
Remember:
Cold, not frozen, is the golden rule.
❌ DON’T: Handle the Sample Directly With Bare Hands
Skin contact can introduce bacteria or contaminants. Always wear sterile gloves when handling the tissue or leave it to your vet.